Figure 4
Sp1 knockdown enhances invasive and migratory abilities of CL1-0 cells. (a) CL1-0 cells were transfected with scrambled or Sp1 small hairpin RNA (shRNA). After incubation for 48 h, cells were harvested for whole-cell lysates and cellular proteins were immunoblotted with anti-Sp1 and actin antibodies. (b) Effect of Sp1 knockdown on invasive ability. The in vitro invasive ability of CL1-0 cells transfected with scrambled or Sp1 shRNA was determined using Matrigel-combined Transwell chambers as described in Materials and methods. Data are representative of three independent experiments and are presented as mean±s.e.m. (*P<0.05). (c) Effect of Sp1 knockdown on the migratory ability measured by wound-healing assay. After transfection with scrambled or Sp1 shRNA for 48 h, confluent monolayers of CL1-0 were wounded and incubated for an additional 24 h. Migratory area was calculated for quantification. Data are representative of three independent experiments and are presented as mean±s.e.m. (*P<0.05). (d) Effect of Sp1 knockdown on the migratory ability examined by Transwell migration assay. The migratory ability of scrambled or Sp1 shRNA-transfected cells was determined by Transwell chambers. Data are representative of three independent experiments and are presented as mean±s.e.m. (*P<0.05).
