Figure 5
Sp1 suppresses lung adenocarcinoma cell metastasis in vivo. (a) After infection of GFP or GFP-Sp1 adenovirus for 48 h, CL1-5 cells ( × 106) were suspended in 100 μl of PBS and injected into the lateral tail vein of severe combined immunodeficient (SCID) mice. After 4 weeks, all mice were killed and the number of pulmonary tumor nodules was calculated 48 h after fixation of lungs with 10% formaldehyde. (Left panel) Representative images of lungs. Arrows indicate pulmonary metastatic tumor nodules. (Central panel) Hematoxylin and eosin (HE) staining of lungs. Arrows indicate pulmonary metastatic tumor. (Right panel) IHC staining using the anti-Sp1 antibody. (b) Confirmation of GFP and GFP-Sp1 expression of CL1-5 cells injected into mice by western blotting. (c) Effects of Sp1 knockdown on in vivo metastasis of CL1-0 cells. (Left panel) Representative images of lungs. (Central panel) HE staining of lungs. (Right panel) Sp1 IHC staining. (d) Confirmation of Sp1 knockdown in CL1-0 cells injected into mice by western blotting. (e, f) Quantitative result (five mice per group) of pulmonary metastatic tumor nodules 4 weeks after injection. Data are expressed as mean±s.e.m. (**P<0.01). (g) Sp1 expression in both CL1-0 and CL1-5 cells that were injected into the tail vein was compared. pSp1 represents phosphorylated Sp1.
