Figure 7 | Oncogene

Figure 7

From: MicroRNA-520/373 family functions as a tumor suppressor in estrogen receptor negative breast cancer by targeting NF-κB and TGF-β signaling pathways

Figure 7

miR-520/373 family inhibits cell invasion in vitro and in vivo intravasation. (a, b) Highly invasive MDA-MB-231 cells transfected with miR-373, miR-520c, si_TGFBR2 or negative control were seeded into trans-well inserts in the presence or absence of TGF-β ligand. The number of invaded cells was determined by flow cytometry (***P<0.001). (c) Number of invaded cells through the trans-well membrane after inhibition of endogenous miR-520c and TGF-β treatment (*P<0.05). Experiments were performed in triplicate and data are shown as mean±s.d. (d, e) Chicken embryo (CAM) assay was performed using MDA-MB-231 cells transfected with miR-373, miR-520c, si_RELA, si_TGFBR2 or negative control. The number of eggs with primary tumor formation for each group is indicated (n). Genomic DNA was analyzed by real-time alu-PCR to determine the amount of human DNA corresponding to human tumor cells that had intravasated to the lower CAM (LCAM). Data were analyzed using Mann–Whitney one-tailed t-test.

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