Figure 3 | Oncogene

Figure 3

From: Ligand-dependent EGFR activation induces the co-expression of IL-6 and PAI-1 via the NFkB pathway in advanced-stage epithelial ovarian cancer

Figure 3

Ligand-dependent EGFR activation induced the production of specific cyto-/chemokines. (a) Fold change in PAI-1 and IL-8 levels compared with unstimulated cells released in media from starved IGROV1 cells stimulated for 4, 8 and 24 h with EGF alone (filled square), EGF plus AG1478 (open square), or AG1478 alone (open circle) evaluated by the Procarta cytokine assay. (b) Real-time RT–PCR for PAI-1 and IL-8 from total RNA of IGROV1 treated as above. Results are presented as relative expression normalized for GAPDH mRNA levels. (c) IL-6, PAI-1 and IL-8 levels measured by the Procarta cytokine assay on conditioned media from starved EOC cell lines left untreated, treated for 24 h with EGF alone, or together with DHMEQ (5 μg/ml). Asterisks indicate a significant difference by one-way analysis of variance (b) and t-test (c).

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