Figure 4 | Oncogene

Figure 4

From: Ligand-dependent EGFR activation induces the co-expression of IL-6 and PAI-1 via the NFkB pathway in advanced-stage epithelial ovarian cancer

Figure 4

IL-6 and PAI-1 expressions are inhibited by EGFR knockdown. (a) Western-blot analysis of total lysates from IGROV1 cells treated with control or EGFR-specific siRNA. The antibodies used are indicated. β-actin is shown as a control for protein loading. A representative experiment is shown. (b) Luciferase promoter gene assay of EGFR-silenced IGROV1 cells transiently transfected with reporter plasmids containing NFkB binding sites. Data are mean values (±s.d.) normalized for transfection efficiency in three independent experiments performed in triplicate. (c) Real-time RT–PCR analysis for EGFR, PAI-1, IL-6 and IL-8 on total RNA from EGFR-silenced IGROV1. Results are represented as relative mRNA expression normalized for GAPDH mRNA levels. (b, c): Asterisks indicate a significant difference by t-test.

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