Figure 2
LNCaP-derived PDGF-D induces pronounced osteoclastic and osteoblastic responses. Sections of intraosseous LNCaP-PDGF-D tumor, LNCaP-neo tumor and control tibia injected with media only were analyzed by H&E, TRAP and trichrome staining, respectively. (a) LNCaP-PDGF-D tumor and its adjacent bone responses occupied the bone marrow cavity shown by H&E staining ( × 100 HE and × 200 HE). The black-boxed area in TRAP staining ( × 100 TRAP) was magnified to × 200 ( × 200 TRAP). Activated osteoclast cells (arrows) at the LNCaP-PDGF-D tumor–bone interface were shown by the dark purple color by TRAP staining. The densely deposited collagen I in the woven bone was shown by the dark blue color by trichrome staining ( × 100). (b) In H&E staining ( × 100 and × 200), a small intraosseous LNCaP-neo tumor circled by blue lines is surrounded by a large sheet of osteoid. The black-boxed area in TRAP staining ( × 100 TRAP) was magnified to × 400. TRAP staining showed very minor staining at the interface between osteoid and native trabecular bone (arrows). Trichrome staining showed that classic basophilic osteoblasts lined the interface between LNCaP-neo tumor (circled by black line) and neighboring osteoid tissue (light blue color). Many osteoblasts were embedded in the osteoid (light blue by trichrome staining) and turning into bone cells. (c) In H&E staining, the media injection-controlled tibia showed normal bone marrow tissue and intact native trabecular bone. TRAP staining showed little to no activated osteoclast cells within bone marrow tissue. Trichrome staining showed the native trabecular bone in dark blue color.
