Figure 5
Osteoprotegerin has little effect on PDGF-D-induced osteoclast differentiation. (a) RAW264.7 cells were treated with 60 ng/ml RANKL or 40 ng/ml PDGF-D in the presence or absence of 50 or 100 ng/ml osteoprotegerin (OPG) for 6 days. An average number of TRAP positive multinucleated cells (more than three nuclei/per cell) in five low-power fields per glass cover slip is shown. *P<0.01. (b) Representative pictures taken after each treatment.
