Figure 6

Processing of full-length PDGF-D is required for the induction of osteoclastic differentiation in RAW264.7 cells. (a) Diagrams of WT or hinge-region mutant (R247, 249A) PDGF-D dimers. (b) RAW264.7 cells were treated with concentrated CM containing WT or mutant PDGF-D for 6 days followed by TRAP staining. Average numbers of differentiated osteoclast cells of treatments were graphed. ^*P<0.01. (c) RAW264.7 cells were treated with CM containing WT or mutant PDGF-D for 3 days. Relative levels of TRAP mRNA after treatments were analyzed by real-time PCR against GAPDH. ^*P<0.01.