Figure 2

Real-time PCR validation of microarray data. Quantitative RT–PCR for selected miRNAs was performed and compared with microarray results. (a, c) miRNA expression across cell lines from microarray and qRT–PCR analyses. Expression in each cell line was normalized to the averaged signal from two melanocyte cell lines. (b, d) Percent change in signal after U0126 treatment from microarray and qRT–PCR analyses, and CI-1040 treatment from qRT–PCR analyses. Values represent average ±s.e.m. from two combined biological replicates (microarray) or three technical replicates (qRT–PCR). The qRT–PCR experiment was performed independently using samples prepared separately from the microarray experiment.