Figure 3 | Oncogene

Figure 3

From: Oncogenic B-Raf signaling in melanoma cells controls a network of microRNAs with combinatorial functions

Figure 3

miRNAs regulated by B-Raf/MKK/ERK control proliferation and 3D invasion of melanoma cells. (a) WM239A cells transfected with 100 nM miRNA were assayed for cell proliferation using WST-1. Values 3 days after plating were normalized to values at the time of plating, and then normalized to DMRIE-C-treated control cells. NT: negative control (non-targeting) miRNA. Error bars represent s.e.m. (n=3). (b) Cell confluency after miRNA transfection. Phase microscopy images were collected on cells transfected as in (a), after 4 days. (c, d) Spheroid growth and invasion following transfection of miRNAs (c) upregulated or (d) downregulated by B-Raf/MKK/ERK.

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