Figure 4

Co-expression of miRNAs enhances suppression of cell invasion. (a) Spheroid invasion following transfection of miRNAs individually and in combination. Cells were transfected with 50 nM of each miRNA mimic and imaged for cell invasion 3 days after collagen implantation. Duplicate spheroids after 3 days in collagen are shown. (b) Cell invasion was quantified by the difference between the outer (R2) and inner (R1) spheroid radii (Supplementary Figure S3, panel a), and normalized to control spheroids transfected with negative control (NT: non-targeting) miRNA. Asterisks indicate individual miRNAs differing significantly from NT (P-value <0.003). Double crosses (‡) designate miRNA combinations that differ significantly from each individual miRNA (P-value<0.05). Error bars represent s.e.m. (n=3).