Figure 1

The AHCreER transgene drives recombination in the gastric epithelium. (a) IHC detecting Cre expression in the antrum of AhCreER⁺ versus AhCreER⁻ mice at day 1 post-induction. Scale bar, 10 μm. (b) IHC detecting Cre expression in the corpus of AhCreER⁺ versus AhCreER⁻ mice at day 1 post-induction. Scale bar, 10 μm. (c) Top: β-Galactosidase staining of whole-mount stomachs from AhCreER⁻ Rosa26^lacZ (left) and AhCreER⁺ Rosa26^lacZ (right) mice at day 4 post-induction. Bottom: In-vivo RFP fluorescence imaging of AhCreER⁻ Rosa26^tdRFP (left) and AhCreER⁺ Rosa26^tdRFP (right) mice at day 4 post-induction (unopened stomach) and at day 10 post-induction (opened stomach). F, forestomach; C, corpus; A, antrum; SI, small intestine; BF, bright field. Forestomach tissue is auto-fluorescent. (d) In-vivo RFP fluorescence imaging of AhCreER⁺ Rosa26^tdRFP (left) and AhCreER⁻ Rosa26^tdRFP (right) mice at day 60 post-induction following a single injection of the inducing agent. Individual gastric pits are positive for RFP (bottom high magnification panel). (e) IHC detecting GFP expression in the stomach of AhCreER⁺ Z/EG mice at day 4 and 20 post-induction. Left two panels, antrum. Right two panels, corpus. Scale bar, 20 μm.