Figure 6
From: SUMOylation of ZFP282 potentiates its positive effect on estrogen signaling in breast tumorigenesis
SUMOylation of ZFP282 positively regulates its co-activator activity and enhances hormone-stimulated breast cancer cell growth. (a) Transient transfection in CV-1 cells using 2ERE-TATA-LUC and expression vectors for ERα, HA-ZFP282 or HA-ZFP282 3KR (200 and 400 ng) were performed, as described in Figure 2a. Data are means±s.d. (n=3). Expression levels of transfected ZFP282 were analyzed by immunoblot with anti-HA antibody. (b) Transient transfection was performed as in (a) with the additional plasmid pSG5.HA-CoCoA (200 ng). Data are means±s.d. (n=3). (c) ChIP and ReIP assay, using the indicated antibodies, was performed, as described in Figure 2e. Data are means±s.d. (n=3). (d) Transient transfection was performed as in (a) using pSG5.HA constructs encoding ZFP282 or SUMO1-fused ZFP282 as indicated. Data are means±s.d. (n=3). Expression levels of transfected ZFP282 were analyzed by immunoblot with anti-HA antibody. (e) Cell proliferation assays were performed, as described in Figure 4b using MCF-7 cells transfected with pSG5.HA-ZFP282 plasmids (WT, 3KR, or SUMO1-fused ZFP282 3KR). Data are means±s.d. (n=6). *P<0.05 and **P<0.01.
