Figure 3
From: Efficient TGFβ-induced epithelial–mesenchymal transition depends on hyaluronan synthase HAS2
Depletion of HAS2 reverts the TGFβ-mediated mesenchymal phenotype. The expression of ZO-1 (green) and F-actin (red) was analyzed by immunofluorescence in NMuMG cells transfected with scramble siRNA or siRNA against either HAS2 (a) or CD44 (b), in unstimulated or TGFβ-stimulated (1 ng/ml, 48 h) cells. Cell nucleus was visualized with DAPI (blue). Images were taken using a Zeiss Axioplan 2 immunofluorescence microscope. Scale bar=20 μm. A representative experiment out of three is shown.
