Figure 2

EphA2 can regulate Erk activation through the phosphorylation of Shp2 and the association of Shp2 with Grb2. (a) Immunoblot analyses with the antibodies indicated at the left using the cell lysates (Total) or the immunoprecipitates with anti-Shp2 (IP: Shp2) prepared from NMuMG (left panel) and HEK293Tcells (right panel) stimulated with HGF (20 ng/ml) for the time indicated on the top. (b) Immunoblot analyses with the antibodies indicated at the left using the cell lysates (Total) or the immunoprecipitates with anti-Shp2 (IP: Shp2) prepared from HEK293T cells tranfected with the plasmids indicated on the top. (c) Immunoblot analyses with the antibodies indicated at the left using the cell lysates (Total) or the immunoprecipitates with anti-Flag (IP: Flag) prepared from HEK293T cells transfected with the plasmids indicated on the top. (d) Immunoblot analyses with the antibodies indicated at the left using the cell lysates (Total) or the immunoprecipitates with anti-Shp2 (IP: Shp2) prepared from HEK293T cells transfected with plasmids indicated on the top and stimulated with HGF for the time indicated on the top. (a–d) Each result is the representative of at least three independent experiments.