Figure 3
EphA2 and disease-associated Shp2 mutants synergistically enhance basal Erk activity in HEK293T cells. (a) Schematic illustration of the domain structure of Shp2 and the amino-acid residues that we examined in this study. SH2, Src homology 2 domain; PTP, protein tyrosine phosphatase domain. (b) Immunoblot analyses with the antibodies indicated at the left using the cell lysates (Total) or the immunoprecipitates with anti-myc (IP: myc) prepared from HEK293T cells transfected with the plasmids indicated on the top. (c) Immunoblot analyses with the antibodies indicated at the left using the cell lysates (Total) or the immunoprecipitates with anti-Flag (IP: Flag) prepared from HEK293T cells transfected with the plasmids indicated on the top. (d) Immunoblot analyses with the antibodies indicated at the left using the cell lysates prepared from HEK293T cells transfected with the plasmids indicated on the top. (e and f) Immunoblot analyses with the antibodies indicated at the left using the cell lysates (Total) or the immunoprecipitates with anti-Flag (IP: Flag) prepared from HEK293T cells transfected with the plasmids indicated on the top. (g–i) Immunoblot analyses with the antibodies indicated at the left using the cell lysates prepared from HEK293T cells transfected with the plasmids indicated on the top. (b–i) Each result is the representative of the three independent experiments.
