Figure 4
Phosphatase-independent and EphA2-dependent Erk activation in Shp2-mediated signal. (a and b) Immunoblot analyses with the antibodies indicated at the left using the cell lysates prepared from HEK293T cells transfected with the plasmids indicated on the top and stimulated with HGF for the time indicated on the top. (c and d) Quantitative analyses of phosphorylation of Erk by HGF in cells expressing empty vector (closed square), Shp2WT-myc, (open square), Shp2T468M-myc (closed circle) or Shp2T468M/2YF (open circle). The relative phosphorylation of Erk was calculated as the value of the intensity of phosphorylated (p)-Erk divided by that of Erk at 30 min in cells transfected with Shp2T468M was 100%. Experiments were performed at least three times. Data are represented as mean with s.d. *P<0.05 among the four groups.
