Figure 5
Tyrosine kinase activity of EphA2 is required for phosphorylation of Shp2 and Erk activation by Shp2. (a) Immunoblot analyses with the antibodies indicated at the left using the cell lysates prepared from HEK293T cells transfected with the plasmids indicated on the top. A representative result of three independent experiments. (b) Immunoblot analyses with the antibodies indicated at the left using the cell lysates prepared from HEK293T cells transfected with the plasmids indicated on the top and treated with Dasatinib (100 nM) or dimethylsulfoxide (DMSO) for the time indicated on the top. (c) Quantitative analysis of (b). The relative Erk phosphorylation after the treatment with Dasatinib was calculated as the intensity of p-Erk divided by that of Erk from the untreated cells at the 0 min was 100%. Data are represented as mean with s.d. *P<0.05 between the cells treated with DMSO and those treated with Dasatinib. (d) Immunoblot analyses with the antibodies indicated at the left using GST-Shp2 incubated with GST-EphA2 for the time indicated on the top.
