Figure 6

Phosphorylation of Shp2 and Erk1/2 in breast cancer expressing EphA2. Immunohistochemical analyses with the antibodies indicated on the top using specimens obtained from normal breast tissue (a), ductal carcinoma in situ (DCIS) (b) and invasive ductal carcinoma (c). Hematoxylin was used for counterstaining. Bar, 100 μm. Positive immunoreactivity is shown as brown color because of detection using 3,3′-diaminobenzidine (DAB) as described in the Materials and methods section.