Figure 1 | Oncogene

Figure 1

From: Stat3-coordinated Lin-28–let-7–HMGA2 and miR-200–ZEB1 circuits initiate and maintain oncostatin M-driven epithelial–mesenchymal transition

Figure 1

OSM is expressed in an autocrine/paracrine fashion in invasive breast carcinoma. (a) The expression of OSM in human breast cancer tissue samples were examined by immunohistochemistry with rabbit polyclonal antibody against OSM. The representative images from the area adjacent to the tumor tissue (left panel) and tumor tissue (right panel) in the same tissue section are shown. (b) The expression levels of OSM and CD68 in breast cancer tissues were detected in serial sections by immunohistochemistry with the anti-OSM antibody and mouse monoclonal antibody against CD68. Bar=25 μM. (c) THP-1 cells were treated with 320 nM PMA (phorbol 12-myristate 13-acetate) for 48 h. For generation of M1- and M2-polarized macrophages, THP-1 cells were treated with 320 nM PMA for 6 h and then cultured with PMA plus 20 ng/ml IL-4 and 20 ng/ml IL-13 (for M2 polarization) or plus 100 ng/ml lipopolysaccharide and 20 ng/ml interferon-γ (for M1 polarization) for another 42 h. The expression of OSM was analyzed by western blot. (d) The expression of OSM and vimentin in human breast cancer cell lines was analyzed by western blot. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

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