Figure 2

Effect of clec14a-CTLD on filopodia formation. (a) HUVECs transfected with GFP, clec14a-GFP or clec14aΔCTLD-GFP were fixed, stained with rhodamine–phalloidin and Hoechst, and examined by confocal microscopy ( × 600). (b) The numbers of filopodia per cell were counted and expressed in a bar graph. (c) The magnified images ( × 2.5) represent the regions of filopodia formation and are indicated by arrows in panel a. Results are representative of three independent experiments.