Figure 7 | Oncogene

Figure 7

From: Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro

Figure 7

Effect of clec14a-CTLD IgGs on clec14a-mediated cell–cell contact. (a) HEK293F cells transfected with GFP and clec14a-GFP were incubated in the absence (MOCK) or presence of clec14a-CTLD IgGs or cetuximab for 8 h. Cell aggregates (mass >4 cells; arrowheads) were counted under a light microscope. The number of aggregates per field is shown in. b. Values represent mean±s.d. of triplicate measurements from one of three independent experiments. (c) HEK293F cells transfected with GFP and clec14a-GFP were subjected to a cell viability assay with a CCK-8 cell counting kit. (d) Following preincubation of hCTLD-Fc with HUVECs, the extent of hCTLD-Fc binding to HUVECs in the absence or presence of increasing concentrations of clec14a-CTLD IgG (clone 1) was measured by cell ELISA. Values represent the mean±s.d. of triplicate measurements from one of three independent experiments.

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