Figure 4

Acetylation of histone H3 lysine 9 indicates that the LMO2 +1 enhancer is active in primary AML patient samples showing activation of the HOXA cluster. (a) ChIP-on-chip analysis of the human LMO2 locus in four patient samples shows a peak of H3 lysine 9 acetylation at the LMO2 +1 enhancer. MVista representation of human/mouse sequence conservation is shown at the top with the +1 enhancer highlighted. Annotations are as in Figure 2a. (b) ChIP-on-chip analysis of the HOXA cluster shows elevated H3 lysine 9 acetylation across central/posterior HOXA genes in the same four patient samples. Enrichment values are calculated as fold enrichment over the mean intensity across the whole locus and expressed as log base 2.