Figure 3 | Oncogene

Figure 3

From: Phospholipase D (PLD) drives cell invasion, tumor growth and metastasis in a human breast cancer xenograph model

Figure 3

A MCF-7 cancer cell line stably overexpressing recombinant human PLD2 shows enhanced cancer cell invasion. (a) Simplified scheme of MIEG-PLD2 mRNA. (b-d) Immunofluorescence of GFP in puromycin-resistant, stable MCF-7 pMIEG cells overexpressing either GFP vector, PLD1 or PLD2. (e) Western blot analyses of cell lysates. (f) Effect of PLD overexpression on cell proliferation. (g) Effect of PLD overexpression on PLD activity. (h, i) Effect of PLD overexpression on physiological functions (cell invasion and chemotaxis, respectively). Triplicate results are mean ±s.e.m. *Denotes statistically significant (P<0.05) increases with respect to controls.

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