Figure 7
The mechanism that regulates PLD-mediated cell invasion and metastasis involves PA, Rac2 and Grb2. (a) PA sensor used for transfection into MDA-MB-231 or MCF-7 cells. (b) Immunofluorescence of PA sensor expression. White arrowheads denote PA sensor localized to a membranous surface that contained PA. (c) Cell invasion of MCF-7 cells stably expressing lipase-inactive constructs (PLD1-K866R or PLD2-K758R). (d) Tumor size of mice injected with MCF-7 cells stably expressing PLD2, a WASp construct or a combination of the two. (e) EGF-mediated cell invasion in cells that overexpress PLD2, Grb2 and Rac2 constructs (WT and SH2-binding deficient). (f) PLD2, Grb2 and Rac2 protein expression in MDA-MB-231 cells. (g) EGF-mediated cell invasion in cells silenced with siRNA for Grb2 and/or PLD2 or with siRNA for Rac2. The symbols * and # denote statistically significant (P<0.05) differences (increases or decreases, respectively) between samples and controls.
