Figure 1 | Oncogene

Figure 1

From: Imiquimod directly inhibits Hedgehog signalling by stimulating adenosine receptor/protein kinase A-mediated GLI phosphorylation

Figure 1

IMQ reduces the Hh/Gli pathway target gene expression in BSZ2, a murine Patched-deficient BCC cell line. (a) IMQ is a synthetic imidazoquinoline used in topical treatment of BCC. (b) The expression levels of two Hh/Gli target genes (Gli1 and Hhip) were measured by quantitative reverse transcriptase–PCR (qRT–PCR) upon IMQ (10 μg/ml=42 μM) treatment. Cyclopamine (Cyc=5 μM) served as a control inhibitor for Hh signalling. Reference gene in all qRT–PCR reactions was Rplp0. (c) Gli1 protein levels of BSZ2 cells after IMQ treatment. Bar chart shows quantification of western blots (b and c: mean values and s.e.m. of two independent experiments).

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