Figure 1
From: p53-dependent gene repression through p21 is mediated by recruitment of E2F4 repression complexes
p21 is required for the downregulation of p53-repression targets. HCT116 WT, HCT116 p53−/−, and HCT116 p21−/− cells were treated with 0.5 μM doxorubicin (Doxo), 20 μM nutlin-3, or vehicle control for 24 h. (a) Cell-cycle analysis. The propidium iodine intensities and the percentages of cells in each cell-cycle phase are indicated. (b, c) Quantitative RT–PCR showing the expression of (b) p53 upregulated target genes and (c) p53-repression target genes. Error bars indicate s.d. of representative experiments performed in triplicate.
