Figure 7
From: p53-dependent gene repression through p21 is mediated by recruitment of E2F4 repression complexes
The p53-repression target ARF is not regulated by p21 or E2F4 repressive complexes. (a) Quantitative RT–PCR showing the expression of ARF in EJp53, EJp21, and EJp16 cells. p53, p21, and p16 were induced in their respective cells by the complete removal of tet for the indicated time points. (b) Quantitative RT–PCR showing the expression of ARF in EJp53 cells stably expressing shGFP or shp21. p53 was induced in by reducing the tet concentration from 1.0 to 0.02 μg/ml. (c, d) ChIP analysis of the indicated genes showing the ratio of E2F4 to E2F1 promoter occupancy in (c) EJp53 cells induced for p53 for 72 h and (d) EJp21 cells induced for p21 for 24 and 48 h. Error bars indicate s.d. of representative experiments performed in triplicate.
