Figure 1
Impaired glycolysis induces MCT1 and CD147 expression. (a) Representative immunoblots and the bar graph represent MCT1 and CD147 protein expression in oxidative SiHa cells cultured during 48 h with the indicated concentrations of glucose, without glutamine, pyruvate, lactate or serum. β-actin was used as a loading control (n=3). (b) SiHa cells were cultured during 24 h in the presence of glucose and of the hexokinase competitor 2-deoxyglucose (2-DG) where indicated. Representative immunoblots and bar graphs represent MCT1 (left) and CD147 (right) protein expression normalized by β-actin (**P<0.01, ***P<0.005 compared to medium with glucose without 2-DG; #P<0.05 compared to medium without glucose and 2-DG; n=3-6). (c) SLC16A1/MCT1 (top) and CD147 (bottom) mRNA in SiHa cells cultured during the indicated times in the presence of glucose and 2-DG where indicated (ns, not significant; n=3). (d) SiHa cells were cultured during 24 h in the presence of glucose and of protein translation inhibitor cycloheximide where indicated. Representative immunoblots are shown for MCT1 and CD147, and the bar graph represents MCT1 protein expression normalized by β-actin. (*P<0.05, **P<0.01 compared to medium with glucose without cycloheximide; n=3). (e) Representative immunoblots and the bar graph show MCT1 (top) and CD147 (bottom) protein expression in SiHa cells cultured during 24 h with glucose and the GAPDH inhibitor 2-iodoacetate (IA) where indicated (*P<0.05, ***P<0.005, n=3).
