Figure 2
From: FOXM1 targets NBS1 to regulate DNA damage-induced senescence and epirubicin resistance
Knockdown of FOXM1 suppresses growth and induces senescence in MCF-7 and MCF-7EpiR cells. (a) MCF-7 and (b) MCF-7EpiR were transfected with NS (non-targeting) siRNA or FOXM1 siRNA. Twenty-four hours after transfection, cells were seeded in six-well plates, treated with epirubicin, grown for 15 days and then stained with crystal violet (left panel). The results (right panel) represent average of three independent experiments±s.d. Statistical significance was determined by Student’s t-test (*P⩽0.05, **P⩽0.01, ***P⩽0.005; n.s., non-significant). In parallel, (c) MCF-7 and (d) MCF-7EpiR transfected with NS siRNA or siFOXM1 were seeded in six-well plates, treated with epirubicin. Five days after treatment, cells were stained for SAβ-gal activity. The graphs c and d show the percentage of SAβ-gal-positive cells as measured from five different fields from two independent experiments. Bars represent average±s.d. Statistical significance was determined by Student’s t-test (*P⩽0.05, **P⩽0.01, ***P⩽0.005, significant; n.s., non-significant).
