Figure 3
From: FOXM1 targets NBS1 to regulate DNA damage-induced senescence and epirubicin resistance
FOXM1 depletion in MCF-7 cells causes the accumulation of significantly higher numbers of γH2AX foci in response to γ-irradiation. (a) MCF-7 and (b) MCF-7EpiR were transfected with NS siRNA or FOXM1 siRNA. Twenty-four hours after transfection, cells cultured on chamber slides were either non-radiated or exposed to 5 Gy of γ-irradiation for 24, 48 and 72 h. Cells were then fixed and immunostained for γH2AX foci (green). Nuclei were counterstained with 4′-6-diamidino-2-phenylindole (DAPI; blue). Images were acquired with Leica TCS SP5 ( × 63 magnification). For each time point, images of at least 100 cells were captured and used for quantification of γH2AX foci number. Results represent average of three independent experiments±s.d. Statistical analyses were conducted using Student’s t-tests against the correspondent time point (**P⩽0.01, significant; n.s., non-significant).
