Figure 7
Podoplanin linkage to the cortical actin cytoskeleton and its association to lipid rafts are required for invadopodia-mediated ECM degradation. (a) Western blot showing the re-expression of WT podoplanin and mutant constructs into podoplanin-depleted HN5 cells. (b, c) The ability of WT podoplanin or the indicated mutant constructs to rescue the invadopodia defects induced by depletion of podoplanin was evaluated by gelatin-degradation assays (6 h). Bar graph represents the percentage of cells forming active invadopodia on each condition. (b) Representative images of the gelatin-degradation assay depicted in b are shown in c. Bars=20 μm. The results shown are the means±s.e.m. of n⩾100 cells for each condition over three independent experiments. ***P<0.0005.
