Figure 2

Expression of miR-206 alters cell cycle kinetics and suppresses cell proliferation, migration and invasion in pancreatic cancer cells. (a) The efficacy of miR-206 mimic overexpression in PANC-1 and PANC10.05 was determined by qRT-PCR. Cell-cycle distribution of PANC-1 or PANC10.05 cells, growing asynchronously and previously transfected with miR-206 mimic or miR-control, was measured by 7-AAD staining and quantified by flow cytometry. Experiments were performed with three replicates and in three independent experiments. (b) Proliferation was evaluated in PANC-1 and PANC10.05 cells for a period of 4 days, using a WST-1 assay. The measured absorbance of cells treated with the miR-control at day 1 was set at 100%. Experiments were performed with six replicates and in three independent experiments. (c and d) End point trans-well migration (c) and matrigel invasion (d) assays of PANC-1 and BxPC-3 cells transfected with microRNA mimics. Cells were transfected, seeded in 0.5% FCS medium and allowed to migrate or invade for 24 h (or 48 h in case of BxPC-3 invasion assay) using full-growth medium as chemoattractant. The number of migrated and invaded cells was quantified by flow cytometry. All experiments were performed with four replicates and in three independent experiments. Data are shown as mean±s.d. and analyzed by a two-tailed unpaired t-test; **P<0.01, ***P<0.001.