Figure 5

Dual-stain FISH analysis of samples that had a c-MYC to chromosome 8 centromere ratio >1.25. Representative FISH images of samples SPO2 (ratio=1.90), SPO28 (ratio=1.28), RAD9 (ratio=2.02) and RAD 10 (ratio=2.50) for combined c-MYC (red) and chromosome 8 centromere (green) hybridisation (a). The results show that c-MYC-amplified cell populations from the radiogenic cohort have a higher degree of amplification compared with amplified cell populations from the sporadic cohort. The heterogeneity of c-MYC and chromosome 8 centromere copy number status is shown in the histograms to the right of the images for each sample (b).