Figure 6 | Oncogene

Figure 6

From: c-MYC is a radiosensitive locus in human breast cells

Figure 6

c-MYC protein expression in radiogenic and sporadic breast cancers. Formalin-fixed, paraffin-embedded tissue from sporadic (n=33) and radiogenic (n=18) breast cancers were sectioned and analysed by immunohistochemistry with a specific c-MYC antibody as described in the Materials and methods. The level of c-MYC expression was quantified by derivation of a histoscore. c-MYC expression in the sporadic and radiogenic breast cancers was compared for all cases (a) and was higher in radiogenic cases in which more than 10% of the nuclei had detectable c-MYC expression (Mann–Whitney U-test: P<0.001) (b). The proportion of sporadic and radiogenic breast cancer that had c-MYC expression histoscores of 0–50, 51–100 and >100 were compared (χ2: P=0.018) (c). The number of samples in each group in panel (c) is identified above each bar of the histogram. c-MYC expression is shown in tumours known to have a c-MYC copy number of <3 (n=22) or ⩾3 (n=7) (d). The wider horizontal bars represent the median c-MYC copy numbers and the narrower horizontal bars represent the 10th and 90th percentiles of the data (a, b and d). Representative IHC images of samples with no c-MYC expression (RAD13: histoscore=0), moderate expression (RAD9: histoscore=70.4) and high expression (RAD6; histoscore=158.8) are shown in (e).

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