Figure 6
From: LARP1 post-transcriptionally regulates mTOR and contributes to cancer progression
LARP1 promotes invasion and anchorage-independent growth. (a) Invasion assay in HeLa cells stably transfected with pTrex-LARP1 and pTrex-LacZ cells or (b) with pooled siRNA to LARP1 and non-targeting control. Graphs are counts of the number of invasive cells per cm2. Representative images of DAPI-stained invasive cells are shown (scale bars, 250 μm). (c) Anchorage-independent growth assay using ultra-low attachment plates in HeLa cells stably transfected with pTrex-LARP1 and pTrex-LacZ. Representative images of spherosomes (scale bars, 200 μm). (d) Number of colonies generated by dissociated spherosomes plated in adherent conditions. Scale bars, 2 cm. Experiments were performed ⩾3 times; data are mean±s.e.m. *P⩽0.05 and **P⩽0.001.
