Figure 1
From: PML is required for telomere stability in non-neoplastic human cells
PML associates with telomeres in telomerase-positive and non-neoplastic cells. (a) Confocal images of a representative immuno-FISH experiment performed with anti-PML antibodies (green, Alexa488) and a telomeric PNA probe (red, Cy3) in normal human fibroblast (MRC5) a telomerase-positive cell line (HeLa) and an ALT cell line (U2OS). PML/Tel colocalizations were identified as overlapping signals in cell nuclei evidenced by DAPI staining. An enlargement of the colocalizing signals pointed by the arrows is shown on the right. (b) Quantitative analysis of immune-FISH experiments for PML-telomere colocalization in WI38, MRC-5 (normal human fibroblasts), mouse embryonic fibroblasts (MEFs), U937, HeLa, A549 (telomerase-positive cancer cells) and U2OS, SK-LU1, SA-OS-2 (ALT-positive cancer cells). Fifty cells per type were scored in each of three experiments. The percentage of PML-NBs co-localizing with telomeres is shown as average ±s.d.
