Figure 1

INPP4B is frequently upregulated in colon cancer cells. (a) Representative microphotographs of immunohistochemical (IHC) staining of INPP4B in tissue microarrays constructed from colon cancers and paired adjacent noncancerous colon tissues. Scale bar, 100 μm. (b) Comparison of INPP4B expression in the 124 colon cancers and paired adjacent noncancerous colon tissues as detailed in Supplementary Table S1. Data are represented as mean immunoreactive score±s.e.m. of IHC staining. ***P<0.001, Kruskal–Wallis test. (c) Comparison of INPP4B mRNA levels of laser capture micro-dissected (LCM) colon cancer cells from the 120 colon cancers and paired adjacent noncancerous epithelial tissues as detailed in Supplementary Table S2. The relative abundance of INPP4B mRNA in the latter was arbitrarily designated as 1. Data are represented as mean±s.e.m. **P<0.01, Student's t-test. (d) Comparison of INPP4B mRNA levels in LCM colon cancer cells (LCM) (n=77) and homogenized colon cancer tissues (HC) (n=17) from the Matsuyama microarray gene expression data set (http://r2.amc.nl). *P<0.05, Student's t-test. (e) Whole-cell lysates from the FHC normal colon epithelial cell line and the indicated colon cancer cell lines with different mutational status of PIK3CA, KRAS and PTEN were subjected to western blotting analysis of INPP4B and GAPDH (as a loading control). Data are representative of three individual experiments. W: wild-type; M: mutant. (f) Total RNA from the FHC normal colon epithelial cell line and the indicated colon cancer cell lines were subjected to qPCR analysis of INPP4B mRNA expression. The relative abundance of INPP4B mRNA in FHC cells was arbitrarily designated as 1. Data are represented as mean±s.e.m. of three individual experiments.