Figure 4
From: Dual-faced SH3BGRL: oncogenic in mice, tumor suppressive in humans
Knockdown of c-Src abrogates mSH3BGRL-induced metastasis in nude mice. (a) Four independent pools of CT-26 SH3BGRL cells stably expressing c-Src-specific shRNA (SH3BGRL-SrcKD 1–4) were analyzed for the expression of c-Src compared with a scrambled control (SH3BGRL-scr). glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as a loading control. (b) Lysates from exponentially growing stable cells (SH3BGRL-SrcKD is a combined pool from all four clones in (a)) were immunoblotted with various antibodies. GAPDH served as a loading control. The averaged relative protein expression level in (a) and (b) is quantified and shown under the immediate panels. (c) In total, 1 × 106 CT-26 SH3BGRL or CT-26 SH3BGRL-SrcKD cells were injected intravenously into the tail vein of nude mice. After 14 days, mice were killed and their lungs were photographed and scored for metastatic tumor nodules (mean±s.d., n=4, ***P<0.001).
