Figure 7
TAE684-resistant SY5Y-TR1 cells are sensitive to HSP90 inhibition. (a) Dose–response curves for parental SH-SY5Y and TAE684-resistant SY5Y-TR1 cells treated with the HSP90 inhibitors 17-AAG and IPI-504 for 3 days (IC50=17-AAG, 650 nM (SH-SY5Y), 61 nM (SY5Y-TR1); IPI-504, 352 nM (SH-SY5Y), 35 nM (SY5Y-TR1)). (b) pRTK array analysis of SY5Y-TR1 cells treated with either DMSO or IPI-504 (1 μM) for 6 h. (c) Western blot analysis of AXL, total and pERK levels in SH-SY5Y and SY5Y-TR1 cells after treatment with 1 μM IPI-504 for the indicated times. (d) Western blot analysis of AXL and HSP90 expression levels in SH-SY5Y and SY5Y-TR1 cells treated with or without 1 μM IPI-504 for 6 h and from which AXL was coimmunoprecipitated with an anti-HSP90 antibody.
