Figure 6
From: Distinct routes to metastasis: plasticity-dependent and plasticity-independent pathways
CS-99 metastasize via MET-dependent pathways. (a) Rint+/Gint− CS-99 cells were mixed 1:1 with CS-99 clones harboring E-cadDipIIIcI2/Gint and injected s.c. CS-99 Rint+/Gint− and E-cadDipIIIcI2/Gint+ cells formed primary tumors with 100% penetrance. (b) Flow-cytometric analysis of the proportion of DsRed+ and GFP+ cells within primary tumors indicates that all primary tumors contain both DsRed+ control cells and E-cadDipIIIcI2/Gint+ cells. (c) Representative images and (d) quantification of CS-99 Rint+/Gint− control cells and E-cadDipIIIcI2/Gint+ suicide reporter cells grown in soft agar. (e) Rint+/Gint− CS-99 cells were mixed 1:1 with CS-99 clones harboring E-cadDipIIIcI2/Gint and injected via the t.v. With the exception of a single GFP+ colony, all metastases were DsRed+ and lacked EGFP expression. (f) Quantification of CS-99 EGFP+ and DsRed+ lung metastases. Each unique symbol represents the number of metastases from a single animal. The median number of Rint+/Gint− and E-cadDipIIIcI2/Gint+ metastases is indicated by a solid black bar.
