Figure 2
From: Disruption of NCOA2 by recurrent fusion with LACTB2 in colorectal cancer
Downregulation of NCOA2 in CRC. (a) The mRNA expression levels of NCOA2, but not LACTB2, were significantly lower in CRC tissues compared with corresponding tumor-adjacent controls as measured by qRT–PCR. (b) Immunohistochemical staining revealed significantly lower NCOA2 protein expression in CRC tissues as compared with corresponding tumor-adjacent tissues. (c) Immunoreactivity of NCOA2 was significantly higher in SW1116 cells overexpressing NCOA2. (d) NCOA2 mRNA levels were much lower in CRC cell lines compared with normal colonic tissues. Results from qRT–PCR and conventional RT–PCR are shown in the chart (top) and as a gel picture (bottom), respectively. Representative micrographs and quantification results of three independent experiments are shown.
