Figure 3
From: Disruption of NCOA2 by recurrent fusion with LACTB2 in colorectal cancer
Tumor-suppressing functions of NCOA2 and their abrogation after fusion with LACTB2 (a) The mRNA expression of full-length NCOA2 and the LACTB2-NCOA2 fusion (LN-fusion) in stably transfected HCT116 and SW1116 cells was confirmed by RT–PCR. The protein expression of full-length NCOA2 after stable transfection was also confirmed by Western blots (WB). (b–e) Enforced expression of full-length NCOA2, but not the LN-fusion, significantly (b) lowered proliferation as measured by xCELLigence System, (c) impaired colony formation, (d) induced early apoptosis and (e) inhibited migration (left) and invasion (right) of HCT116 and SW1116 cells. (f) The growth of HCT116 xenografts stably expressing NCOA2 was significantly slower than those stably transfected with empty vector or LN-fusion in nude mice. Data are represented as mean±s.d. of three separate experiments in triplicates. *P<0.05; **P<0.01; ***P<0.005 significantly different between indicated groups.
