Figure 4
From: Disruption of NCOA2 by recurrent fusion with LACTB2 in colorectal cancer
Pro-tumorigenic effects of NCOA2 knockdown in normal colonocytes NCM460. (a) The downregulation of mRNA and protein expression of endogenous NCOA2 on transfection with siRNA was confirmed by RT–PCR and Western blots (WB), respectively. (b) Proliferation of NCOA2-knocked down NCM460 cells was accelerated when compared with control siRNA-transfected cells. (c) Basal apoptosis of NCM460 cells was significantly reduced on transfection with NCOA2 siRNA. Assays were conducted 48 h post transfection and data are represented as mean±s.d. of three separate experiments in triplicates. *P<0.05; **P<0.01 significantly different between indicated groups.
