Figure 5
From: Disruption of NCOA2 by recurrent fusion with LACTB2 in colorectal cancer
Repression of Wnt/β-catenin by wild-type NCOA2 but not the LACTB2-NOCA2 fusion (LN-fusion) protein in CRC cells. (a and b) Western blots and luciferase reporter activity show (a) the reduction of active β-catenin and (b) the inhibition of β-catenin transcriptional activity on enforced expression of NCOA2, but not the LN-fusion protein, in stably transfected HCT116 cells. (c) Stable overexpression of NCOA2, but not the LN-fusion protein, caused dramatic alterations in the expression of Wnt signaling components in HCT116 cells as measured by pathway-specific RT–PCR array. (d) The concurrent upregulation of Wnt inhibitors (for example, APC and Frzb) and downregulation of Wnt stimulators (for example, Frizzled, CK1 and Dvl) as well as the repression of β-catenin transcriptional targets (that is, FOSL1 and Jun) on stable expression of NOCA2 in HCT116 cells were illustrated in the schematic diagram. Gel pictures are representative and data are represented as mean±s.d. of three independent experiments. *P<0.05 significantly different between indicated groups.
