Figure 2 | Oncogene

Figure 2

From: The BET inhibitor JQ1 selectively impairs tumour response to hypoxia and downregulates CA9 and angiogenesis in triple negative breast cancer

Figure 2

JQ1 reduces TNBC monolayer and spheroid growth, regardless of MYC. (a) Cell cultures were grown in 96-well plates for 72 h in each condition. (+)-JQ1 dose-dependently reduces monolayer (two-dimensional) cell growth of TNBC cell lines MDA-MB-231 and HCC1806 in normoxia and hypoxia, while (−)-JQ1 does not. (b) JQ1 reduced c-Myc immunocontent only in the MYC-amplified cell line HCC1806, while the non-mutated cell line MDA-MB-231 showed no difference in c-Myc. (c) Representative spheroid growth curve and pictures of MDA-MB-231 spheroids following JQ1 treatment. (d) (+)-JQ1 reduces spheroid growth in MDA-MB-231 and other TNBC cell lines, while (−)-JQ1 does not. One-way analysis of variance, n=3, *P<0.05, **P<0.01, ***P<0.001.

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