Figure 2 | Oncogene

Figure 2

From: The ERK–ZEB1 pathway mediates epithelial–mesenchymal transition in pemetrexed resistant lung cancer cells with suppression by vinca alkaloids

Figure 2

Investigation of the pemetrexed-induced metastasis by a mice metastasis model. The cells of A549/A400 and A549 were tail-vein injected into mice followed by monitoring for 55 days until sacrifice. The lung tissues were examined for tumor metastasis and the weights of the two groups were recorded and listed (a). H&E and IHC of TS staining were applied to verify the tumors and TS overexpression followed by pERK, ZEB1, E cadherin and fibronectin detection (b). All five CL1-injected mice were free of tumors and two of the CL1/A200-injected mice established metastatic tumors, which were stained with H&E and IHC with anti-TS antibody (c).

Back to article page