Figure 3

Induction of NKG2D-Ls by HDACis was dependent on the acetyltransferases CBP/p300. (a) Intracellular FACS analysis of HEK-293 cells stained for acetylated lysine residues. Cells were preincubated with or without 75 μM of the histone acetyltransferase inhibitor (HATi) anacardic acid (ANAC) or 10 μM of the p300 (and CBP) inhibitor C646 for 3 h and subsequently treated with 100 nM LBH589 for 1 h. (b) Flow cytometric analysis of HEK-293 cells treated with or without 8 μM C646 and 10 μM Ara-C or 250 nM trichostatin A (TSA) for 16 h. (c) HEK-293 cells were incubated with 50 μM ANAC, 8 μM C646 or 10 μM CBP-CREB interaction inhibitor (KIXi) and treated with 100 nM LBH589 (LBH). (d) Real-time PCR of HEK-293 cells preincubated with or without 10 μM C646 for 3 h and treated with 100 nM LBH589 for 1 h. MICA, MICB and ULBP2 mRNA expression levels were analyzed relative to GAPDH.