Figure 7
From: CBP/p300 acetyltransferases regulate the expression of NKG2D ligands on tumor cells
CBP/p300 were crucial for the expression of RAE-1 in vivo. (a) Eμ-Myc CBP/P300 littermates show deletion of either CBP or p300. Genomic DNA extracted from tumor cells of terminally ill Eμ-Myc CBP/P300 double mutants (Bnull) was subjected to PCR using specific primers to detect recombined (Δflox) or non-recombined (flox) genes. Representative examples are shown. (b) Flow cytometric analysis of tumor cells from lymph nodes to detect MULT1 and RAE-1 (right panel) and of tumor cells from lymph nodes (tumor), spleen or peripheral blood (PB) (left panel) isolated from Eμ-Myc mice (ctrl) or Eμ-Myc with CBP/p300-deficient B cells (Bnull). (c) Real-time PCR to detect RAE-1 transcripts expressed in tumor cells from Eμ-Myc mice (ctrl) or Eμ-Myc with CBP/p300-deficient B cells (Bnull). RAE-1 mRNA expression was analyzed relative to HPRT. (d) Flow cytometric analysis of MCA-205 cells that were preincubated with 8 μM C646 and treated with 5 nM LBH589 for 16 h followed by detection of MULT1 and RAE-1 surface expression using flow cytometry.
