Figure 2 | Oncogene

Figure 2

From: The Snail repressor recruits EZH2 to specific genomic sites through the enrollment of the lncRNA HOTAIR in epithelial-to-mesenchymal transition

Figure 2

HOTAIR has a functional role in EMT. (a) RT–qPCR analysis for the indicated markers on HOTAIR-silenced hepatocytes (siHotair), compared with control siGFP cells (siCtr), treated (TGFβ) or not (Ctr) with TGFβ for 24 h. The values are calculated by the 2(−ΔCt) method, expressed as fold of expression versus the control (arbitrary value=1) and shown as means±s.e.m. Statistically significant differences are reported (*P<0.05, **P<0.01) for five independent experiments (NS=no significance). (b) RT–qPCR analysis for the same markers as in (a) on hepatocytes overexpressing Snail (Snail), or a control vector (Ctr), and both HOTAIR (siHotair) or GFP (siCtr) silenced. RNAs were collected 72 h after infection and 48 h after transfection. The values are calculated as in (a). Statistically significant differences are reported (*P<0.05, **P<0.01) for five independent experiments. (c) Phase-contrast and immunofluorescence analysis for the indicated markers in cells as in (b) (magnification × 20). Blue DAPI staining shows the nuclei (DNA).

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